Characterizing phenotypes of CG15365 mutants in Drosophila melanogaster

Characterizing phenotypes of CG15365 mutants in Drosophila melanogaster

The gene CG15365 in Drosophila melanogaster has an unknown function, and its potential role in biological processes remains unexplored. We hypothesized CG15365 to be orthologous to the human gene LZTS1, a gene implicated in cell cycle regulation and cancer development. More recently, LZTS1 has been implicated in the development of hypermobile Ehlers-Danlos syndrome. To investigate this possibility, a series of computational analyses, including DNA, mRNA, and amino acid sequence alignments as well as structural comparisons, were performed to evaluate potential functional similarities between CG15365 and LZTS1. Our findings suggest that CG15365 is not orthologous to LZTS1, raising new questions about its biological role. We aimed to further investigate the function of CG15365 in Drosophila melanogaster. Climbing assays were conducted with Drosophila melanogaster models to assess the impact of CG15365 loss-of-function on neuromuscular function. Results revealed no significant difference in the crawling velocity of CG15365 mutants compared to wild-type models, leaving questions about the function of CG15365. In the future, we plan to perform viability assays to understand any role CG15365 mutation may play in development. Understanding the role of CG15365 may provide insights into its potential relevance to genetic mechanisms in the model organism Drosophila melanogaster, which could have broader implications for understanding gene functions in other species.